I've made "poorman's" microfluidics by cutting pieces of scotch tape on glass slides and casting PDMS against it. However, the cells I'm working with are small and motile enough to make anything created this way totally inadequate. Besides, I'm a bit worried that the random organics in PDMS (and Scotch tape adhesive) can throw off my cellular response.
So here's what I want to test - an agar/agarose pad with a groove patterned it in connected to a Laplace pressure pump. More specifics:
- I can make a dozen of these pads (at least ones without a groove) in about an hour
- To make the groove I need to pattern the slide I use to 'flatten' the pad
- The Laplace pump will be made with a coverslip with a tiny hole in it that:
- I place a drop of stimulant over
- will be far away from where I image cells
- needs to be aligned to the groove in the pad